What is the proper biosafety level for this suite?

• A. BSL-1
• B. BSL-2
• C. BSL-3
• D. BSL-4

Answer: (C)

Handling agents that can cause serious, potentially life-threatening disease and can be transmitted through the air requires stronger containment. Biosafety levels scale the minimum practices, equipment, and facilities needed for different risk categories. A suite designated for work with more dangerous pathogens that pose an inhalation risk is set up at the BSL-3 level. In this environment, access is controlled, the laboratory is under negative pressure, and exhaust air is HEPA-filtered to prevent release of aerosols. Procedures that can generate aerosols are conducted inside protective containment like biological safety cabinets, and personnel wear appropriate respiratory protection. This level sits between the basics of lower levels, which cover less risky organisms, and the highest level, which is reserved for the most dangerous pathogens requiring full-body, dedicated facilities. So, the suite is equipped to safely handle serious agents with inhalation risk, which is why BSL-3 is the proper choice.